Table 1.
FISH signal details in BCR-ABL1 positive leukemia patients
| CML-CP (n = 243) | CML-BP (n = 17) | BCR-ABL1+ ALL (n = 52) | |
|---|---|---|---|
| FISH signals | 1R1G1F;1R2G1F;2R1G1F; 2R2G1F;1R1G2F;1R1G3F | 1R1G1F;1R1G2F;1R1G3F;1G4F; 1R2G1F;1R2G2F;1R2G3F;2R2G1F; 2R1G2F; 2R2G2F | 1R1G1F;1R1G2F;1R1G3F; 1G4F;1R2G1F;1R2G2F;2R1G1F; 2R2G1F;1G3F;2G3F; 1R4F;1R1G4F |
| Complex signal patterns | 1R1G2F/2R1G1F(n = 1) | 1R1G1F/1R2G1F(n = 1) | 1R1G2F/1R1G4F/1R1G3F (n = 1) |
| 1R1G2F/2R2G1F(n = 2) | 1R2G1F/1R1G2F(n = 1) | 1R1G2F/1R1G3F/1R2G2F (n = 1) | |
| 1R1G2F/1R1G1F(n = 1) | 1G4F/1R1G2F (n = 1) | 1R1G2F/1R1G3F/1G3F/2R4F | |
| 1R1G1F/2R1G1F(n = 1) | 1R1G2F/1R2G2F/1R2G3F (n = 1) | (n = 1) | |
| 2R1G2F/2R2G2F (n = 1) | 1R1G3F/1R1G2F (n = 6) | ||
| 1R1G1F/1R2G2F (n = 1) | 1R1G3F/1R1G2F/1G3F n = 1) | ||
| 1R1G2F/1R1G3F (n = 3) | 1R2G2F/1RnG2F (n = 1) | ||
| 1R1G2F/1R1G4F (n = 1) | |||
|
2G3F/3F (n = 1) 1R1G2F/1G4F/2G8F (n = 1) | |||
| 1R1G2F/2R2G1F (n = 1) | |||
| 1R1G2F/1R2G1F (n = 1) | |||
| Typical single patterns | 1R1G2F (n = 179) | 1R1G2F (n = 5) | 1R1G2F (n = 28) |
| Atypical single patterns | 2R1G1F (n = 16) | 1R1G1F (n = 1) | 2R2G1F (n = 1) |
| 1R2G1F (n = 8) | 1R1G3F(n = 2) | 1R1G1F (n = 3) | |
| 1R1G1F (n = 20) | 1G3F (n = 1) | ||
| 2R2G1F (n = 14) | 1R1G3F (n = 2) | ||
| 1R1G3F (n = 1) | 2R1G1F (n = 1) |
Some BCR-ABL1 signal patterns and their interpretations (R = red signal; G = green signal; F = fusion signal) [21]
1R1G2F: t(9;22)
1R2G1F: t(9;22) with deletion of the derivative chromosome 9 involving only the sequences 5′ of the ABL1 breakpoint
2R1G1F: t(9;22) with deletion of the derivative chromosome 9 involving only the chromosome 22 sequences 3′ of the BCR breakpoint
1R1G1F: t(9;22) with deletion of the derivative chromosome 9 involving sequences 5′ of the ABL1 breakpoint as well the chromosome 22 sequences 3′ of the BCR breakpoint
1R1G3F: t(9;22) with additonal Philadelphia chromosome
2R2G1F: Variant (three-or four-way) t(9;22)
1RnG2F: nG represents many added green signals that we are different to count