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. 2019 Oct 7;38:413. doi: 10.1186/s13046-019-1415-5

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — CyclinD1 and H3K9me3 are collocated in the nucleus and form a complex with SUV39H1, HP1α and DNMTs in ICC cells. a Western blot analysis reveals that CyclinD1 and H3K9me3 are mainly expressed in the nucleus in HuccT-1 and HCCC9810 cells. b Immunofluorescence (IF) analysis of the nuclear co-localization of CyclinD1 with H3K9me3 in HuccT-1 and HCCC9810 cells (400×, magnification). c-f Anti-CyclinD1 immunoprecipitates endogenously with SUV39H1, H3K9me3 and HP1α from HuccT-1, HCCC9810 and HIBEpic cells