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. 2019 Sep 16;218(10):3223–3236. doi: 10.1083/jcb.201811060

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© 2019 Asai et al.

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Figure 5. — The SETβ signal leaves centromeres in metaphase. (A) HeLa cells released from RO3306 and then treated with colcemid (top and B–D), with MG132 (bottom and H and I), or not treated (middle and E–G) were fixed and stained with anti-SETβ (green), Sgo2 (red), and Cenp-C (blue) antibodies. DAPI was used to visualize DNA. Kinetochore pairs in white enclosures are shown in large scale. Scale bars, 5 μm. (B–I) Line scans of individual sister kinetochores/centromeres in the white enclosures are shown from left to right.