Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 5;218(10):3212–3222. doi: 10.1083/jcb.201907178

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Pineda et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — The Hras mutant epithelium nonautonomously impacts the mesenchyme and follicular regeneration. All epithelial nuclei are in green (K14H2BGFP), and recombined cells are in red (tdTomato). (A) Top: Two-photon revisits reveal that K19CreER;Hras^G12V/+ follicles reenter the growth phase more rapidly than K19CreER;Hras^+/+ controls. Follicles are labeled A–C for ease of recognition, and cycling follicles are labeled with an asterisk. Bottom: Quantification of follicle staging over an 8-wk (W1–W8) period after tamoxifen induction (n = 1,704 follicles across 4 Hras^+/+ mice and n = 1,413 follicles across 3 Hras^G12V/+ mice). (B) Top: Cartoon schematic depicting the cycling and noncycling regions of the ear skin and imaged region. Bottom: Two-photon images of the hair follicles in the permanently quiescent region of skin at the tip of the ear in Hras^+/+ and Hras^G12V/+ mice show that while wild-type follicles do not cycle, Hras^G12V/+ follicles do (observed in five K19CreER;Hras^G12V/+ mice). TAM, tamoxifen. (C) In utero injection cartoon that depicts targeted lentiviral-Cre delivery to the amniotic sack at embryonic day 9.5. Injection with lentiviral-Cre into the Hras^G12V/+;K14H2BGFP;tdTomato system directly tags and activates Hras^G12V/+ cells in a mosaic fashion. (D) Two-photon images of Hras^+/+ (left) and Hras^G12V/+ (right) hair follicles during the “second rest phase” (P55). Mosaic activation of Hras using lentiviral-Cre delivery demonstrates that wild-type follicles (green) within the Hras^G12V/+ skin reenter the growth phase precociously along with their Hras mutant (red) neighbors while follicles in the Hras^+/+ mouse remain appropriately in rest phase (observed in n = 2 LV-Cre Hras^G12V/+ mice compared with n = 3 LV-Cre Hras^+/+ mice). (E) Cartoon summary depicting the enhanced regenerative power of Hras^G12V/+ follicles in both cycling and noncycling skin. All scale bars represent 50 µm.