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. 2019 Sep 5;218(10):3212–3222. doi: 10.1083/jcb.201907178

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© 2019 Pineda et al.

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Figure 4. — Follicles are resistant to oncogenic growth, even in the face of additional tumor promoting insults. All epithelial nuclei are in green (K14H2BGFP), and recombined cells are in red (tdTomato). (A) Cartoon schematic detailing the various approaches for testing the buffering effect. (B–D) Two-photon revisits show that targeted ablation of the bulb region in Hras^+/+ (B), Hras^G12V/+ (C), and Hras^G12V/+;TGFβ^fl/fl (D) follicles during the growth phase results in follicular deformation and subsequent regeneration. (E) Mutant follicles rapidly regenerate after injury, and within 1 mo after ablation, ∼75% of mutant follicles are cycling again compared with only 35% of wild-type follicles (n = 23 Hras^+/+ follicles across two mice, n = 34 Hras^G12V/+ across four mice, n = 19 Hras^G12V/+;TGFβ^fl/fl follicles across three mice). All error bars indicate standard deviation. (F) Cartoon summary depicting rapid, normal regeneration of Hras^G12V/+ after injury. All scale bars represent 50 µm.