Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 19;218(10):3436–3454. doi: 10.1083/jcb.201903109

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Klapproth et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — Reduced kindlin-3 expression impairs leupaxin recruitment to podosomes. (A) IF staining of a K3^−/− preosteoclast retrovirally transduced with EGFP-K3 (green), for leupaxin (red) and F-actin (white/blue in merge). (B) IF stainings of K3^+/+ and K3^n/− preosteoclasts for paxillin (green), leupaxin (red), and actin (white/blue in merge). (C) Western blot analyses on lysates of K3^+/+, K3^+/n, K3^n/n, and K3^n/− macrophages for leupaxin, paxillin, PTP-PEST, and talin expression. (D) Densitometric quantification of leupaxin expression in K3^n/− macrophages relative to WT cells. All values were normalized to the corresponding GAPDH signal. n = 6. Scale bars, 10 µm. Dotted white lines mark cell borders.