Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 19;218(10):3436–3454. doi: 10.1083/jcb.201903109

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Klapproth et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 8. — Characterization of podosomes from paxillin/leupaxin double deficient RAW cells. (A) Western blot analyses of +/+ RAW cells and four different clones of paxillin/leupaxin dKO RAW cells for their expression of kindlin-3 and talin. (B and C) IF stainings of +/+ and paxillin/leupaxin dKO RAW cells for vinculin (green), integrin β1 (red, B), kindin-3 (red, C) and actin (white/blue in merge). (D) Diameter of the podosome actin cores in +/+ and paxillin/leupaxin dKO RAW cells. 10 actin cores in two regions of five to eight cells were measured in each experiment. n = 9/7. (E) Control RAW cells and different clones of paxillin/leupaxin dKO RAW cells were transfected with LifeAct-GFP and imaged at a spinning disc microscope for 10 min with a 15-s time interval. The cumulative distribution of these measurements is shown. 20 podosomes were measured per cell. At least four cells were analyzed in each of six dishes. (F) IF stainings for talin (green), tyrosine-phosphorylated proteins (red) and actin (white/blue in merge) on +/+, paxillin/leupaxin dKO, paxillin^−/− and leupaxin^−/− RAW cells. (G) Fluorescence intensity profiles of actin (blue) and phospho-tyrosine (p-Y; red) through three actin cores (indicated by the white lines in E) of +/+, paxillin/leupaxin dKO, paxillin^−/−, and leupaxin^−/− RAW cells. (H) Western blot analyses of the phosphorylation status of paxillin, Pyk2, FAK and cortactin in +/+, paxillin^−/−, leupaxin^−/−, K3^−/− and paxillin/leupaxin dKO RAW cells kept in suspension or adherent to fibronectin. Scale bars, 10 µm. Dotted white lines mark cell borders.