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(A) MDCK and MDCK-B4GalNT2 were infected with PR8 and its PB2 mutants at MOI 1 for 8 h. Mean ± sd, n = 3. (B) MDCK and MDCK-B4GalNT2were infected with WSN, Dk/Bav, Cal04, HK09, 4167, OK323, Ar02 and OK309 at MOI 4 for 8 h. Mean ± 1sd, n = 3. (C) Proportion of NP positive cells normalized to that of wild type MDCK. Mean ± sd, n = 2. *p < 0.05, **p < 0.01, ***p < 0.001, 2 tailed T-test.
