Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 27;18(5):4367–4376. doi: 10.3892/ol.2019.10785

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Vayalil et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 3. — Measurement of mitochondrial bioenergetic function using the XF24 analyzer and determination of different mitochondrial bioenergetic parameters from OCR profile. Seahorse Bioscience V7 Tissue Culture Plates were seeded with optimal cell density and allowed to grow for 24 h prior to the measurement of OCR. Basal OCR was measured three times followed by the sequential addition of Oligo, FCCP and AntiA with a measurement of OCR. Colored boxes in the OCR trace represent the relative contribution of basal oxygen consumption, non-respiratory chain oxygen consumption, ATP-linked oxygen consumption, the maximal OCR after the addition of FCCP, proton leak and the reserve capacity of the cells. AntiA, antimycin A; FCCP, carbonyl cyanide-p-trifluoromethoxyphenylhydrazone; OCR, oxygen consumption rate; Oligo, oligomycin.