. 2019 Sep 14;11(9):476. doi: 10.3390/pharmaceutics11090476

Table 2.

List of the formulations selected for transconjunctival permeation experiments, composition of the receptor medium, and of the mixtures used for drug extraction from tissue.

Drug	Donor		Receptor Phase	Extraction Mixture
Drug	Vehicle	Drug Conc. (mg/mL)	Receptor Phase	Extraction Mixture
Cyclosporine	T	ss ^a	PBS + TPGS 1 mM ^b	CH₃CN: 1% CH₃COOH (87:13)
Cyclosporine	Miglyol^® (solution)	5	PBS + TPGS 1 mM ^b	CH₃CN: 1% CH₃COOH (87:13)
Dexamethasone	T ^c	ss ^a	PBS ^d	CH₃CN: water (35:65)
	P	ss ^a	PBS ^d
	Miglyol^® (dispersion)	1.5	PBS ^d
Econazole Nitrate	TP	ss ^a	PBS + TPGS 0.2 mM ^e	CH₃CN: pH 4 buffer (60:40)
	P	ss ^a	PBS + TPGS 0.2 mM ^e
	T	ss ^a	PBS + TPGS 0.2 mM ^e
	T	ss ^a	PBS + 1% BSA ^f
	Miglyol^® (dispersion)	3.8	PBS + 1% BSA ^f

^a ss: saturated solution; see Table 4 for values. ^b Cyclosporine solubility: 290 ± 10 µg/mL. ^c In this condition, also the transport of TPGS across the conjunctiva was evaluated. ^d Dexamethasone solubility: 79 ± 4 µg/mL. ^e Econazole nitrate solubility: 91 ± 8 µg/mL; ^f Econazole nitrate solubility: 245 ± 29 µg/mL. BSA was precipitated before analysis by adding 260 µL of CH₃CN and 40 µL of HClO₄ to the receptor samples (300 µL); the recovery efficiency was evaluated and resulted >85%.