Table 3.
HPLC-UV methods for the different analytes.
| Parameter | Cyclosporine | Dexamethasone | Econazole Nitrate | TPGS |
|---|---|---|---|---|
| Column (Waters, Milford, MA, USA) | Nova-Pack C18
(150 × 3.9 mm, 4 µm) |
Nova-Pack C18
(150 × 3.9 mm, 4 µm) |
XTerra RP18 (4.6 × 100 mm, 3.5 µm) |
C18 Simmetry 300 (250 × 4.6 mm, 5 µm) |
| Oven temperature (°C) | 65 | 45 | 37 | 40 |
| Mobile phase | CH3CN: water with TFA 0.1% (65:35 v/v) | CH3CN: water (35:65 v/v) |
CH3CN: pH 4 acetate buffer (70:30 v/v) | MeOH: pH 5.8 acetate buffer (97:3 v/v) |
| Flow (mL/min) | 1.6 | 1.0 | 1.0 | 2.0 |
| Absorption wavelength (nm) | 230 | 240 | 223 | 215 |
| Retention time (min) | ~5 | ~3 | ~2.5 | ~4 |