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. 2019 Oct 1;10:2336. doi: 10.3389/fimmu.2019.02336

Figure 3.

Figure 3

Autoantibody production in wildtype mice of diverse genetic backgrounds exposed to silica. (A) Anti-ssDNA IgM; (B) Anti-ssDNA IgG; (C) IgM concentration; and (D) Anti-MPO IgM levels in bronchoalveolar lavage fluid (BALF) from wildtype mice 1–3 months after exposure to silica or vehicle. Autoantibody levels were measured as OD405 for binding to antigen minus binding to diluent-only using undiluted BALF in duplicate. (E) Anti-ssDNA IgG levels in supernatants derived from lung cells cultured with a mixture of TLR7 and TLR9 ligands R848 and CpG oligos, from mice of indicated exposure and strain. (F) Anti-ssDNA IgG levels in serum diluted 1/100 or 1/500, as indicated. Each symbol represents an individual mouse; the median for each group is indicated by the bar; *p < 0.05 and **p < 0.01 for silica- vs. vehicle-exposed mice of same strain, Wilcoxon rank sum test.