. 2019 Oct 1;10:2336. doi: 10.3389/fimmu.2019.02336

Table 1.

Differences between silica-exposed wildtype mice of diverse autoimmune genetic backgrounds.

	Si-exposed strain								Kruskal-Wallis			Steel-Dwass all pairs
	B6		BXSB		MRL		NZB		Chi square	df	p-value	p-value
Parameter	Median	IQR^a	Median	IQR	Median	IQR	Median	IQR
BALF LEUKOCYTE COUNT (THOUSANDS)^b
	275	322	1,772	1124	1,096	2,406	147	185	17.77	3	0.0005	<0.05, BXSB vs. B6 & NZB
												<0.05, MRL vs. B6 & NZB
LUNG CD45+ LEUKOCYTE COUNT (MILLIONS)^b
	4.6	12.6	2.3	0.7	5.9	7.3	4.0	16.8	11.62	3	0.0088	<0.05, BXSB vs. MRL & NZB
LUNG TLS COUNT^c
	51	34	29	28	70	46	14.5	20.3	15.55	3	0.0014	<0.05, MRL vs. BXSB
												<0.01, MRL vs. NZB
LUNG TLS AREA %^c
	0.79	0.84	1.11	1.76	1.47	0.91	0.47	1.23	10.03	3	0.0183	<0.05, MRL vs. B6
BALF autoAb LEVELS (OD405)^d
α-DNA IgM	0.262	0.255	0.965	0.928	1.386	1.223	0.635	0.340	19.85	3	0.0002	<0.01, B6 vs. MRL
												<0.05, B6 vs. BXSB & NZB
α-DNA IgG	0.058	0.056	0.445	0.661	1.510	1.452	0.273	0.616	20.63	3	0.0001	<0.01, B6 vs. MRL & BXSB
α-MPO IgM	0.000	0.005	0.079	0.094	0.003	0.047	0.008	0.026	12.97	3	0.0047	<0.01, B6 vs. BXSB
LUNG CELL CULTURE (TLR LIGAND STIMULATED) ANTI-DNA IgG (OD405)^d
TLR7/9	0.011	0.029	–	–	0.312	0.910	0.000	0.013	16.10	2	0.0003	<0.01, MRL vs. B6 & NZB
TLR4	0.000	0.003	–	–	0.100	0.307	0.000	0.014	16.08	2	0.0003	<0.001, MRL vs. B6
												<0.05, MRL vs. NZB
SERUM ANTI-DNA IgG (OD405)^d
	0.553	0.572	0.278	0.362	2.965	2.536	2.045	1.727	21.35	3	<0.0001	<0.01, MRL vs. B6 & BXSB
												<0.05, NZB vs. BXSB

BALF, bronchoalveolar lavage fluid; IQR, interquartile range; df, degrees of freedom; MPO, myeloperoxidase; Si, silica; TLR, toll-like receptor; TLS, tertiary lymphoid structures.

BALF leukocyte counts were determined by Cellometer K2 and lung counts by flow cytometry.

Lung TLS count and % area were quantified on gridded images of scanned whole lung sections stained for B and T cells.

Autoantibodies in undiluted BALF and lung cell culture supernatants and in sera diluted 1:100, except for MRL sera diluted 1:500, were measured using ELISA.