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. 2019 Oct 1;6:213. doi: 10.3389/fmed.2019.00213

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Copyright © 2019 Sigdel, Nguyen, Liberto, Dobi, Junger, Vincenti, Laszik and Sarwal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell specific gene expression of CXCL9 and CXCL10 by chromogenic in situ hybridization (ciSH) data agrees with gene expression data on bulk tissue. Representative images from biopsy samples with T-cell mediated rejection. Chromogenic in situ hybridization for CXCL9 (A) and CXCL10 (B) shows high-level expression on tubular epithelial cells (asterisks) and some scattered mononuclear cells (arrow), 400×. Rare signal was noted in some glomeruli, however no definite signal was detected in the vascular compartment or in the interstitium other than the inflammatory cells (32, 33).