TABLE 2.
Oligonucleotide real-time qPCR primers.
| Gene | Accession numbera | Primer sequence (5′→3′) | Orientation | Product size (bp) |
| HBA1 | NM_001004376 | TCCATGCTTCCCTGGACAA | Forward | 59 |
| GTACTTGGCGGTCAGCACAGT | Reverse | |||
| HBBR | NM_001004390 | CCGAGGAGAAGCAGCTCATC | Forward | 65 |
| TTCGGCACCGCATTCC | Reverse | |||
| HBM | NM_001004375 | GAGCAACCTGCATGCCTACA | Forward | 59 |
| GCGACAACAGCTTGAAATTGAC | Reverse | |||
| HBZ | NM_001004374 | TGCCGTGACCACCATCTG | Forward | 56 |
| CCAGCCCAATGGACTCAATC | Reverse | |||
| HBE | NM_001081704 | TCCTGCCTGCCAATTTGC | Forward | 55 |
| CAGAGCATGAGCCACAACGT | Reverse | |||
| FPN1 | BM486402 | CGCATAAGGCTAGCGCTTTC | Forward | 62 |
| GTGTTGCCTTCCCCGACTT | Reverse | |||
| FTH1 | NM_205086 | CCACGAGGAGCGTGAACAT | Forward | 58 |
| TCCACCCCTCTGGTTTTGC | Reverse | |||
| FTL | NM_204383 | TGCTGGAGCTCGCCTACAG | Forward | 60 |
| CCACGTGTGACTGATCAAAATATTC | Reverse | |||
| HEPH | XM_420165 | GGACTGGAATTATGCTCCAACAG | Forward | 68 |
| CCTTTAGGCTACGTGTGATGCTT | Reverse | |||
| HJV | XM_025143560 | GCTCCGGATCACCAAAGCT | Forward | 61 |
| AGCGGAACGTCTTCTCGTAGTC | Reverse | |||
| MB | NM_00116775 | GGCAGCACTTGAGACCTATCTATCT | Forward | 59 |
| TCGCTGAGCCCCATGGT | Reverse | |||
| TFR2 | NM_205256 | ACCTTGGAACTGGAGACCCTTAC | Forward | 64 |
| GGTGGAAACTGGGTGTGGTT | Reverse | |||
| HIFPH2 | XM_015284393 | CGCCGCAACCCTCATG | Forward | 64 |
| AATACCACACTGTTATTGCGTACCTT | reverse | |||
| Akt | AF039943 | TTCAACGGTGATCTTTTGACTGA | Forward | 64 |
| CGGGAATGTCTCTTGGTGGAT | Reverse | |||
| HIF-1 α | NM_204297 | AACACACCATGATATGTTCACGAAA | Forward | 83 |
| CCCAGACGTAGCCACCTTGT | Reverse | |||
| PI3Kα | NM_001004410 | GCCATCTTACTCCAGGCGTATC | Forward | 70 |
| GAGGGACTTGGCTGTAGCTTCTC | Reverse | |||
| 18S | AF173612 | TCCCCTCCCGTTACTTGGAT | Forward | 60 |
| GCGCTCGTCGGCATGTA | Reverse |
aAccession number refers to GenBank (NCBI). AKT, V-Akt murine thymoma viral oncogene homolog or protein kinase B (PKB); HBA1, hemoglobin subunit alpha 1; HBE, hemoglobin subunit epsilon; HBBR, hemoglobin beta, subunit rho; HBM, hemoglobin subunit mu; HBZ, hemoglobin subunit zeta; HEPH, hephaestin; HIF-1α, hypoxia inducible factor 1 alpha; HIFPH2, hypoxia-inducible factor prolyl hydroxylase 2; HJV, hemojuvelin; FTH1, ferritin heavy chain 1; FPN1, ferroportin 1; FTL, ferritin light chain; MB, myoglobin; PI3K, phosphatidylinositol 3-kinase; TFR2, transferrin receptor 2.