Figure 2.

HT29 and HCT116 cells contain differential levels of SNX3, which is inversely associated with their migratory and invasive potential. (A) Transwell assays were performed to determine the migratory and invasive abilities of HT29 and HCT116 cells. Scale bar, 200 µm. (B) Number of cells that had invaded or migrated to the lower membrane surface in each field after 48 h was counted. (C) Representative images of the wound healing assay were obtained at 0 and 24 h after scratching. Scale bar, 50 µm. (D) Western blotting was performed to examine the expression levels of SNX3 and epithelial-mesenchymal transition-associated proteins in the colorectal cancer cell lines HCT116 and HT29. GAPDH was used as the loading control. (E) Protein expression levels of E-cadherin and vimentin were assessed by immunofluorescence in HT29 and HCT116 cells. Vimentin (red), E-cadherin (red) and DAPI (blue); scale bar, 50 µm. **P<0.01; ***P<0.001. SNX3, sorting nexin 3.