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. 2019 Sep 12;18(5):5332–5340. doi: 10.3892/ol.2019.10860

Figure 4.

Figure 4.

SNX3 knockdown promotes the migration and invasion of HT29 cells by promoting EMT. (A) Protein expression levels of SNX3 were detected by western blotting. GAPDH was used as the loading control. (B) Transwell assays were performed to determine the migratory and invasive abilities of NC-HT29 and si-SNX3-HT29 cells. Scale bar, 100 µm. (C) Number of cells that had invaded or migrated to the lower membrane surface in each field after 48 h was counted. (D) Western blotting was performed to examine the expression levels of EMT-associated proteins in NC-HT29 and si-SNX3-HT29 cells. GAPDH was used as the loading control. (E) Protein expression levels of E-cadherin and vimentin were detected by immunofluorescence in NC-HT29 and si-SNX3-HT29 cells. Vimentin (red), E-cadherin (red) and DAPI (blue); scale bar, 50 µm. *P<0.05; **P<0.01; ***P<0.001. EMT, epithelial-mesenchymal transition; NC, negative control; si/siRNA, small interfering RNA; SNX3, sorting nexin 3.