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. 2019 Sep 20;18(5):5399–5407. doi: 10.3892/ol.2019.10903

Figure 3.

Figure 3.

PD-1/PD-L1 inhibitor restores the function of lymphocytes. (A) Jurkat cells transfected with PGL3-NFAT-TA-Luciferase plasmid cocultured with various cancer cell lines were treated with A0-L (10 µM) and subsequently stimulated with anti-CD3 (1 µg/ml) and anti-CD28 (1 µg/ml). Luciferase activity was measured 24 h later. **P<0.01, ***P<0.001. hPBMCs were co-cultured with MCF-7 or MDA-MB-231 in the presence of A0-L (10 µM), subsequently stimulated with anti-CD3 (1 µg/ml) and anti-CD28 (1 µg/ml), and the levels of (B) IL-2 and (C) IFN-γ were measured. ***P<0.001. PD-1, programmed cell death 1; PD-L1, programmed death ligand 1; IL-2, interleukin-2; IFN-γ, interferon-γ; hPBMC, human peripheral blood mononuclear cells.