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. 2019 Oct 1;10:2162. doi: 10.3389/fimmu.2019.02162

Figure 1.

Figure 1

(A) Shows Irf4 mRNA-relative expression (normalized per 18S rRNA) of wild type (WT) tubular epithelial cells (TECs), differentiated CD11c+ and CD11b+ bone marrow–derived cells under resting conditions. (B) Time course of Irf4 mRNA induction following LPS (100 ng/ml) stimulation in cultured TECs (white) and bone marrow–derived myeloid cells (dark gray). (C) Time course of Irf4 mRNA and protein (western blot) expression from whole kidney tissues after induction of unilateral ischemia reperfusion injury (IRI) up to 5 weeks post-IRI. (D) Irf4 mRNA expression from intrarenal parenchymal cells (white bar, flow-through of magnetic cell sorting technique (MACS) isolation system), CD11c+ cells (light gray), and CD11b+ (dark gray) bars. *p < 0.05. (E) F4/80-stained macrophages in kidneys of WT 10 days, 3, and 5 weeks post–unilateral IRI induction. The numbers of macrophages were quantitated per HPF; n = 12 per group were examined. Data are shown as mean ± SEM. **p < 0.01; ***p < 0.001.