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. 2019 Oct 1;10:2162. doi: 10.3389/fimmu.2019.02162

Figure 5.

Figure 5

Unilateral IRI was induced in 4- to 6-week-old WT (white bars) and IRF4-deficient mice (black bars). Operated kidneys were harvested 21 days after induction of the model. CD11b+ cells were isolated from kidneys by MACS magnetic separation. Cells were checked for purity and lysed for RNA extraction. RT-PCR of the indicated genes was performed and normalized per 18S rRNA. *p < 0.05.