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. 2019 Oct 1;10:2320. doi: 10.3389/fimmu.2019.02320

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Copyright © 2019 Erpenbeck, Gruhn, Kudryasheva, Günay, Meyer, Busse, Neubert, Schön, Rehfeldt and Kruss.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NETosis depends on substrate elasticity, stimulant, and surface coating. Human neutrophils were seeded on PAA gels and glass surfaces coated with either collagen I (A,B) or fibrinogen (C,D), and stimulated with either PMA (5 nM) (A,C) or LPS (75 μg/mL) (B,D) and incubated for 3 h. PMA-induced NETosis does not depend on substrate elasticity. LPS-induced NETosis increases significantly for both coatings above a stiffness threshold of >20 kPa. n > 500 cells for each condition. N = 3 donors. Statistics: one-way ANOVA (Bonferroni's multiple comparisons test; *p < 0.05; **p < 0.01; ****p < 0.0001; ns: not significant). Mean ± SEM of independent donors.