Figure 6.

LPS-induced NETosis but not PMA-induced NETosis requires adhesion. RICM images of fixed cells cultured on glass (A), PLL (B), and PLL-g-PEG (C) coated glass surfaces. Compared to glass and PLL surfaces, neutrophils cultured on PLL-g-PEG show no adhesion (C). NETosis on these different substrates was quantified for PMA (5 nM) (D), and LPS (150 μg/ml) stimulation (E). PMA-induced NETosis also took place on the passivated non-adhesive PLL-g-PEG surfaces, while LPS-induced NETosis did not occur. n > 500 cells for each condition. N = 3 donors. Statistics: One-way ANOVA (Bonferroni's multiple comparisons test **p < 0.01; ns: not significant). Mean ± SEM of independent donors.