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. 2019 Jul 29;213(2):501–515. doi: 10.1534/genetics.119.302477

Figure 3.

Figure 3

Regulation of the subcellular localization of YAP-1 in intestine. (A) Subcellular localization of intestinal YAP-1 in developing worms. Nuclear (arrowhead) and membranous (arrows) localization of YAP-1 was restricted to late embryos and early L1-stage worms. From L1 stage, intestinal YAP-1 was consistently localized in the cytoplasm. (B) WTS-1-mediated regulation of subcellular localization of YAP-1 in intestine. Nucleus was labeled with SUR-5::mCherry. Knockdown of WTS-1 by RNAi caused nuclear (arrowheads) and membranous (arrow) localization of intestinal YAP-1 in larva. Right graph: quantified results. “cytosplasmic”: worms that show exclusive expression of YAP-1::GFP in cytoplasm; “intermediate (L, low)”: worms that show nuclear and membranous YAP-1::GFP only in anterior intestine; “intermediate (M, medium)”: worms that have nuclear and membranous YAP-1::GFP in anterior intestine and some other intestinal cells; “nuclear + membranous”: worms that show nuclear and membranous YAP-1::GFP throughout the entire intestine. Data: average percentages of worms ± SD. Bar, 10 μm.