Table 1. Summary of 10 × Genomics Single-cell RNA Sequencing.

Summary of barcodes and sequencing parameters	U87MG	U87MG-EGFRvIII
Estimated number of cells	9,365	6,763
Fraction reads in cells	80.9%	75.9%
Mean reads per cell	56,095	92,544
Median genes per cell	3,890	4,238
Total genes detected	16,094	15,874
Median UMI counts per cell	20,033	26,811
Reads mapped confidently to the transcriptome	66.6%	69.5%
Reads mapped confidently to exonic regions	70.8%	73.5%
Reads mapped confidently to intronic regions	13.2%	10.5%
Reads mapped confidently to intergenic regions	5.8%	4.5%
Reads mapped antisense to the gene	5.2%	5.0%