Table 1. Primer sequence.
Gene | Sequence |
miR-93 | F: 5′- AGTCTCTGGCTGACTACATCACAG -3′ |
R: 5′- CTACTCACAAAACAGGAGTGGAATC -3′ | |
LIMK1 | F: 5′- GGGGCATCATCA AGAGCA-3′ |
R: 5′- CCAGGCAGTTGTGGGAGT-3′ | |
RhoA | F: 5′- TCGGAATGATGAGCACACAA-3′ |
R: 5′- GCTTCACAAGATGAGGCAC-3′ | |
ROCK1 | F: 5′- GTGATGGCTATTATGGACG-3′ |
R: 5′- AGGAAGGCACAAATGAGAT-3′ | |
U6 | F: 5′CTCGCTTCGGCAGCACATATACT3′ |
R: 5′ACGCTTCACGAATTTGCGTGTC3′ | |
GAPDH | F: 5′- ACGGCAAGTTCAACGGCACAG -3′ |
R: 5′- GACGCCAGTAGACTCCACGACA -3′ |
Note: F, forward; R, reverse; LIMK1, LIM domain kinase 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.