Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 4;12:8105–8115. doi: 10.2147/OTT.S223423

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Hu et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Exogenous ADHFE1 knockdown promotes the proliferation of CRC cells, and the upregulation of ADHFE1 inhibits the proliferation of CRC cells. (A) Expression analyses of ADHFE1 protein in different CRC cells using western. GAPDH was used as a loading control. (B) Western blot analysis of the overexpression and knockdown of ADHFE1 in CRC cell lines. GAPDH was used as a loading control. (C and D) CCK8 analyses of the CRC cell proliferation with ADHFE1 overexpression or knockdown. (E and F) Colony formation analyses of the CRC cell proliferation with ADHFE1 overexpression or knockdown. (G) The xenograft models were generated after injecting SW480/ShNC and SW480/ShADHFE1 cells in nude mice (n = 6/group). The tumor volumes were measured on the indicated days. The data points represent the mean tumor volumes ± SD. (H) The sections of tumor were subjected to H&E staining or IHC staining using an antibody against Ki-67. Error bars represent the means ± SD from three independent experiments. **p<0.01, ***p<0.001, ****p<0.0001.