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. 2019 Jul 9;220(10):1589–1598. doi: 10.1093/infdis/jiz357

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© The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Opsonophagocytic killing activity of anti glycoconjugate rabbit sera against prototype enterococcal strains. Enterococcus faecalis type 2 (A) and vancomycin-resistant Enterococcus faecium 11236/1 (B) were tested with antibodies raised against the conjugates DHG-PpiC (horizontal stripes) and DHG-SagA (vertical stripes), used at the same titer concentration ratio. The absolute immunoglobulin G (IgG) concentration of the sera is shown in the x-axis. Sera raised against the native DHG (black bar) and the recombinant proteins PpiC (diagonal stripes) and SagA (square grids) were used as controls. The effectiveness of opsonophagocytic killing by the anti-conjugate rabbit sera obtained from terminal immune sera (terminal) was compared to that by the preimmune rabbit sera (preimmune; lighter color). Comparisons of preimmune and terminal immune sera at the same concentration were made by the nonparametric Kruskal-Wallis test, followed by the Dunn multiple comparisons post hoc test. Bars and whiskers denote mean values ± standard errors of the mean. NS, not significant (P ≥ .05). *P < .05, **P < .01, ***P < .001, and ****P < .0001.