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. 2019 Jul 9;220(10):1589–1598. doi: 10.1093/infdis/jiz357

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© The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Opsonophagocytic assay of anti-glycoconjugate rabbit sera against different enterococcal strains. The opsonophagocytic activity was tested against Enterococcus faecalis V583 (A), E. faecalis type 5 (B), Enterococcus faecium E155 (C), and E. faecium 757875 (D). The antibodies raised against DHG-PpiC (horizontal stripes) and DHG-SagA (vertical stripes) were used at the same titer concentration ratio. Serum raised against the native DHG (black bars) and the recombinant proteins PpiC (diagonal stripes) and SagA (square grids) were used as controls. Effective opsonophagocytic killing in the α-DHG-protein rabbit sera from terminal immune sera (terminal) was compared to preimmune rabbit sera (preimmune; lighter color) by the nonparametric Kruskal-Wallis test, followed by the Dunn multiple comparisons post hoc test. Bars and whiskers denote mean values ± standard errors of the mean. NS, not significant (P ≥ .05). *P < .05, **P < .01, ***P < .001, and ****P < .0001.