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. 2019 Sep 26;17(9):e3000453. doi: 10.1371/journal.pbio.3000453

Fig 4. Population WNT levels determine distribution patterns of single-cell G1 length.

Fig 4

(A and B) Western blot of β-catenin in nuclear (n = 3) and total (n = 4) fractions of H9 cells. (C) qPCR analysis of WNT target genes in H9 cells grown either in E8 or in mTeSR1 (n = 6). (D) Histograms for G1 length of H9 cells grown in mTeSR1 and treated with recombinant human WNT3A proteins (100 ng/ml) (n = 114 for mTeSR1 and n = 104 for mTeSR1 + WNT3A pooled from three independent experiments); U test: p-value < 2.2 × 10−16, KS test: p-value = 2.492 × 10−11. (E) Histograms for G1 length of H9 cells grown in E8 and treated with IWP-2 (5 μM) (n = 112 for E8 and n = 104 for E8 + IWP-2 pooled from three independent experiments); U test: p-value = 8.282 × 10−12, KS test: p-value = 1.167 × 10−9. Underlying data can be found in S1 Data. AXIN2, Axis inhibition protein 2; C-MYC, c-myc proto-oncogene; CV, coefficient of variation; E8, Essential 8; KS, Kolmogorov-Smirnov; N-MYC, n-myc proto-oncogene; n.s., not significant; OCT4, Octamer-binding transcription factor 4; qPCR, quantitative PCR; Stdev, standard deviation; WNT, Wingless-INT.