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. 2019 Sep 26;17(9):e3000453. doi: 10.1371/journal.pbio.3000453

Fig 5. A Poisson model predicts single-cell distribution patterns of G1 length based on population WNT levels.

Fig 5

(A) Correlation between AXIN2 expression and the dose of recombinant human WNT3A proteins added to H9 cells in mTeSR1 (n = 4). (B) Relationship between average G1 length and population AXIN2 expression in H9 cells treated with various doses of recombinant human WNT3A proteins (n = 114 for 0 ng/ml, n = 42 for 10 ng/ml, n = 48 for 20 ng/ml, and n = 104 for 100 ng/ml pooled from two to three independent experiments). Error bars represent SEM. (C) Poisson regression trained with the data in Fig 5B. (D) Poisson regression analysis for combined hESCs and hiPSCs. G1 length is average of multiple single-cell G1 lengths. AXIN2 expression was measured by qPCR (n = 4). Underlying data can be found in S1 Data. AXIN2, Axis inhibition protein 2; E8, Essential 8; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GLM, generalized linear model; hESC, human embryonic stem cell; hiPSC, human induced pluripotent stem cell; qPCR, quantitative PCR; WNT, Wingless-INT.