Fig. 4.

EGTA-AM (50 μm), a Ca²⁺ chelator with slow Ca²⁺-binding kinetics, enhanced the fEPSP amplitude in slices from aged animals but did not attenuate fEPSPs when applied to young-mature brain slices for 45 min. Top, Single recordings from young and aged slices during control, EGTA-AM application, and washout. Bottom, Barsindicate mean ± SE. Numbers inparentheses represent the number of slices in each group. *p < 0.01, significant difference from baseline; paired Student’s t test. Note the significant effect that EGTA-AM had on the fEPSPs, but not on the presynaptic volleys, in aged slices (arrows).