Fig. 6.

Open-field test. A, Spontaneous locomotor activity of wild-type and mGluR7^−/−knockout mice (n = 9) was determined by the total amount of activity during each 1 min time period after the animals had been placed in a novel open-field environment. ANOVA with repeated measures indicated no reliable difference in genotype (F_(1,16) = 0.32, p = 0.58) or interaction between genotype and time (F_(29,464) = 0.69, p = 0.89) in locomotor activity. B, Mean numbers of defecations and urinations of the two genotypes (n= 18) were determined for the first 30 min after the animals had been placed in a novel open-field environment. Data points and bars represent mean ± SEM, respectively. There was no statistical difference in any of the tests between two genotypes by Student’st test: for defecations (t₍₃₄₎ = 0.85, p = 0.40) and urinations (t₍₃₄₎ = −0.52,p = 0.61). In both A andB, mGluR7^−/− knockout and wild-type mice generated by back-crossing into the C57BL/6 genetic background for seven generations were used.