Fig. 3.

Effect of 8Br-cAMP or forskolin on cAMP production and APP synthesis in cultured astrocytes. A, Cellular cAMP levels in astrocytes stimulated by 8Br-cAMP (250 μm) or forskolin (50 μm). Graph represents means and SEM from a representative experiment performed on duplicate dishes (*p < 0.05). B, Representative Northern and Western blots show that APP mRNA, APP holoprotein (Holo APP), and GFAP levels are increased by 8Br-cAMP (250 μm) or forskolin (50 μm). The amount of RNA loaded for each lane on Northern blots was not different as measured by G3PDH mRNA levels.