Fig. 5.

Effect of the immunosuppressant cyclosporin A or FK-506 on APP synthesis caused by PGE₂ or forskolin treatment of cultured astrocytes. A, Increases in APP mRNA caused by PGE₂ (10 μm) are inhibited by the immunosuppressant cyclosporin A (CsA) or FK-506 (both 50 μm). Graph and SEM represent data collected from three independent experiments (*p < 0.05).B, Increases in APP holoprotein caused by forskolin (50 μm) are inhibited by the immunosuppressant cyclosporin A (CsA) or FK-506 (both 50 μm). Graph and SEM represent data collected from three independent experiments (*p < 0.05). C, Representative Northern and Western blots show that the increases in APP mRNA and APP holoprotein stimulated by PGE₂ (10 μm), but not the increases in GFAP levels, were inhibited by cyclosporin A (CsA) or FK-506 (both 50 μm).