Table 1.
Comparison of the antigenic phenotypes of astrocyte precursor cells and perinatal astrocytes
APCs | Astrocytes | |
---|---|---|
Pax2 | + | + |
GFAP | − | + |
S100β | − | + |
A2B5 | + | − |
C5 | + | + |
RAN-2 | + | + |
Vimentin | + | + |
Nestin | ± | ± |
Purified E17 APCs and P1 astrocytes were cultured for 1 hr and immunostained by antibodies against each of the above markers. + indicates >90% of the cells were positive, and − indicates <10% of the cells were positive. Values in between these were considered ±. Approximately 40% of the APCs and astrocytes were nestin+.