Fig. 3.
Properties of the recombinant α1P250T receptor protein. A, Western blot of membrane preparations from HEK 293. Immunostaining by monoclonal antibody mAb 4a, which specifically recognizes GlyR α subunits, produced no detectable differences between cells transfected with the wild-type (wt) or the mutated (P250T) α1 cDNA construct.B, Ligand-binding properties of recombinant GlyR α1 and α1P250T receptors. Values present displacement of [3H]strychnine binding by unlabeled strychnine and glycine.