Fig. 2.

Glutathione content in astroglia–neuron coculture. The neurons were incubated in DMEM/PS in the absence (open circles) or the presence (closed circles) of cell culture inserts containing astroglial cells. The glutathione content was determined at the time points indicated in the neuronal (A, closed circles) and astroglial (B) compartment of the coculture, in neuron-rich cultures incubated in the absence of astroglial cells (A, open circles), and in the incubation medium of the coculture (C). At the onset of the incubation, the primary cultures of neurons and astroglial cells were 6- and 21-d-old, respectively. The data presented are triplicates ± SD obtained in one experiment. Using independent cultures, the experiment was repeated twice with comparable results.