Fig. 10.

Double immunostaining of COX-2 (red) and von Willebrand factor (green), an endothelial cell marker in a large subarachnoidal blood vessel (A), in a small parenchymal blood vessel (B), and in a large parenchymal blood vessel (C1, C2). An additional staining for nucleus was done with TOTO-3 (blue) in C2, and the magnified views were shown inD1 and D2. Note that COX-2-IR structure overlapped in a large part with nuclear staining resulting inpinkish color. Double immunostaining of COX-2 (red) and OX-42 (green) provided little evidence for the expression of COX-2 in microglial/macrophages (E). Scale bars: A, B, 10 μm; C1, C2, 25 μm; D1,D2, 7.5 μm; E, 15 μm.