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Q35-GFP expression results in the formation of many small aggregates per cell, whereasQ80-GFP expression results in the formation of a few large aggregates per cell. At 5 DIV, cerebellar granule neurons were transfected with Q35-GFP or Q80-GFP. The number of aggregates per cell was counted (A), and the diameter of each aggregate was measured (B) 4 d after transfection. Error bars represent SEM; n > 50.
