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Western blot of CD23 from SN of a patient with PD after SDS-PAGE, blotting on nitrocellulose membranes, and immunodetection with 135 mAb against CD23. After revelation by enhanced chemiluminescence, two major bands at 45 and 43 kDa were observed together with minor bands ranging from 41 to 67 kDa (lane 1). Treatment of tissue homogenate by enzymatic deglycosylation revealed a new band at 36 kDa, corresponding to the molecular weight deduced from the peptidic sequence of CD23 (lane 2).
