Double-immunofluorescence staining of CD23 and glial markers in the SN of patients with PD. GFAP and ferritin were used as markers of astrocytes and microglial cells, respectively. Positive astrocytes are indicated by arrows inA, and microglial cells are indicated byarrowheads in B. On sections stained simultaneously for GFAP (A) and ferritin (B), no cells were stained for both markers. On double-stained sections, CD23 (D,F) was detected in astrocytes (C) and some microglial cells (E). Microglial cells expressing CD23 are indicated by an arrowhead in E andF. Scale bar, 20 μm.