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. 1999 May 1;19(9):3404–3413. doi: 10.1523/JNEUROSCI.19-09-03404.1999

Fig. 6.

Fig. 6.

Chimeric MBP transcripts containing ETn sequences are abundantly expressed in the CNS and PNS of the lesrat. A, Schematic of PCR strategy used to identify chimeric mRNA detected in les rats. Approximate locations of primers in MBP exons 1 and 3 and the LTR of the ETn element used in PCR are indicated by half arrows. Cloning and sequencing of the PCR products revealed that they consisted of Mbp exons 1 and/or 3 fused to portions ofMbp intron 3 and ETn sequences. B, Autoradiograms of Northern blots in which RNA (20 μg) extracted from brains of 4-week-old mutant (les) and control (C) rats was hybridized with32P-labeled probes specific either for one of the chimericles PCR products (left;Mbp exon 3–intron 3–ETn) or a 362 bpEcoRI fragment of the ETn element (right).