Fig. 2.

Western blot analysis of cytosolic cytochrome c from wild-type mice. Top, Cytochrome c (Cyt. c) from the cytosolic fraction in the control and nonischemic brains (lane 1 on theleft), 24 hr after ischemia (lane 2), and in the ischemic brains (lanes 3–6), with 3.6 μg of protein loaded per lane. Cytochrome c immunoreactivity was evident as a single band of molecular mass 15 kDa in the cytosolic fraction in the ischemic brain as early as 1 hr after ischemia (lane 3), whereas it was barely detected in both the normal control brain and the contralateral brain. A time-dependent increase of cytosolic cytochrome c was observed.Bottom, The result of the β-actin analysis shown as an internal control. The results shown are representative of two independent studies. C, Control brain.