Fig. 6.

Silencing of ICER in rat pinealocytes primarily diminishes inducibility in ICER immunoreactivity. Top panels, NE induces nuclear pCREB (a) and ICER (b) immunoreactivity in isolated pinealocytes (compare columns 1 and 2).Bottom panels, The SUMDENS values from semiquantitative image analyses (n = 12) corrected for the total area covered by the cells [corrSUMDENS (Wicht et al., 1999)] are shown. Similar results were obtained with the β₁-adrenergic agonist isoproterenol (data not shown).Column 1, Unstimulated pinealocytes (control).a, The NE-induced increase in pCREB immunoreactivity in pinealocyte preparations (compare columns 1 and2) is independent of transfected DNA (comparecolumns 2 and 3–5). b, Silencing ICER (column 5, pICERas) decreases NE-induced ICER immunoreactivity, as compared with that of NE-stimulated pinealocytes that were untransfected (column 2) or transfected with control DNA (column 3, pRcCMV) or with pICERs (column 4).