Fig. 1.

NGF-induced apoptosis of mature oligodendrocytes is blocked by the caspase inhibitors YVAD-CHO and DEVD-CHO. Confocal microscopy of untreated (A) or NGF-treated (B) oligodendrocyte cultures immunostained with antibodies against the specific oligodendrocyte marker O1 (red) and TUNEL (green) are shown. Immunofluorescence microscopy of cultures treated with 100 μm DMSO (C), YVAD-CHO (E), or DEVD-CHO (G) in the absence of NGF (C, E,G) or the presence of 100 ng/ml NGF for 4 hr (D, F, H). The arrows in D indicate TUNEL-positive nuclei in NGF–DMSO-treated cultures. Scale bar: A,B, 10 μm; C–H, 25 μm.