Fig. 4.

Caspase-1, -2, and -3 are processed in NGF-treated cultured oligodendrocytes. Total cell lysates (50 μg) from either untreated oligodendrocyte cultures (lanes 1,3, 5) or NGF-treated cultures (lanes 2, 4, 6) were loaded on a 15% SDS-PAGE gel, Western blotted with anti-caspase-1 antibody ICEp10(M-20) (lanes 1, 2), anti-caspase-3 antibody CSP-3 (lanes 3,4), or anti-caspase-2 antibody Nedd-2p12 (lanes 5, 6). The top panels indicate the procaspase forms indicated as proenzyme. The middle panels are the processed forms (p10 or p20 subunit) of the caspases, indicated by the arrows. The bottom panels are internal controls for α-actin levels.