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. 1999 Apr 15;19(8):3043–3049. doi: 10.1523/JNEUROSCI.19-08-03043.1999

Fig. 5.

Fig. 5.

In situimmunodetection of activated caspase-3 (CPP32) in cultured oligodendrocytes. Confocal microscopy of oligodendrocyte cultures double-stained with the mature oligodendrocyte-specific marker O1 (red) and the CM1 antibody (Srinivasan et al., 1998b), specific for the p18 subunit of the activated caspase-3 (green). The activated form of caspase-3 was not observed in untreated culture (A). Activated caspases can be seen in the cell body of O1-positive cells after 4 (B) and 8 (C) hr of NGF treatment. Arrows indicate cell bodies. Scale bar:A–C, 10 μm.