Table 1.
Summary of experimental conditions
| Study 1 | Study 2 | Study 3 | Totals | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Perfused | Early slices | Late slices (set 1) | Late slices (set 2) | Perfused | Late slices | Perfused | Late slices | ||
| Experimental conditions | |||||||||
| Animals (n) | 2 | 3 | 5 | 2 | 2 | 2 | 2 | 2 | 20 |
| Age (d) | 39, 77 | 60–65 | 55–63 | 53, 60 | 68, 68 | 65, 66 | 21, 22 | 21, 22 | — |
| Weight (gm) | 137, 310 | 279–310 | 246–310 | 236, 279 | 337, 411 | 334, 355 | 41, 55 | 52, 61 | — |
| Fixative (% glutaraldehyde) | 2.5 | 6.0 | 6.0 | 6.0 | 6.0 | 6.0 | 6.0 | 6.0 | — |
| Time in vitro(hr) | — | 1.75–2.4 | 6–13 | 4.5–5.75 | — | 9–10 | — | 9–10 | — |
| Number of series | 5 | 4 | 6 | 12 | 2 | 2 | 2 | 2 | 35 |
| Serial sections per series | 27–68 | 25–58 | 26–58 | 33–38 | 98–106 | 107–109 | 112–134 | 95–112 | 25–134 |
| Section Thickness (nm) | 55–80 | 58–83 | 59–77 | 49–71 | 64–65 | 50–60 | 54–65 | 56 | 49–83 |
| ASD analysis | |||||||||
| SA (μm2) | 1044 | 469 | 715 | — | — | — | — | — | 2228 |
| HNA (μm2) | 836 | 301 | 595 | — | — | — | — | — | 1732 |
| Total synapses (n) | 465 | 256 | 395 | — | — | — | — | — | 1116 |
| Dendrite analysis | |||||||||
| Dendrites (n) | 52 | — | — | 129 | 16 | 24 | 21 | 12 | 254 |
| Dendrite Diameter (μm) | 0.62 ± 0.016 | — | — | 0.62 ± 0.015 | 0.53 ± 0.02 | 0.59 ± 0.02 | 0.55 ± 0.02 | 0.52 ± 0.05 | — |
| Total Length (μm) | 155 | — | — | 254 | 107 | 125 | 155 | 68 | 864 |
| Total Spine Synapses (n) | 543 | — | — | 1914 | 376 | 548 | 332 | 270 | 3983 |
| Total Shaft Synapses (n) | 20 | — | — | 63 | 57 | 31 | 44 | 14 | 229 |
| Total Synapses (n) | 563 | — | — | 1977 | 433 | 579 | 376 | 284 | 4212 |
Number of series, The number of photographic series that were examined in each condition; Serial sections per series, the range in the number of sections that were in a particular series. For example, in Study 1, Late Slices (set 2), there ranged from 33 to 38 serial sections in each of the 12 series.