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BDNF promotes the expression of ARPP-21 in high-density cultures. A, E13-derived neurons plated at 1 × 106 cells/cm2 form large aggregates after 7 DIV but remain ARPP-21-negative.B, Treatment with BDNF (50 ng/ml) for the entire 7 DIV results in a marked increase in ARPP-21 immunopositivity. Magnification, 20×.
