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. 1999 Jul 1;19(13):5409–5419. doi: 10.1523/JNEUROSCI.19-13-05409.1999

Fig. 2.

Fig. 2.

Neurobasal/B27 promotes the differentiation of a subset of MSNs. The percentage of DARPP-32-positive neurons is increased in E13- and E17-derived low-density (1 × 105 cells/cm2) cultures grown in Neurobasal/B27 in the absence of exogenous growth factors relative to cultures grown at high density, in serum, or in the presence of dopamine. The percentage of DARPP-32-positive cells was calculated after 7 DIV, but the numbers in high-density (HD) cultures are approximate because of the difficulty of counting positive numbers of cells in aggregates. The percentage represents the average of fields from the center and the periphery of the cultures. The percentage in HD (1 × 106 cells/cm2) and in RF (defined medium plus 10% FCS) is greater than in DM plus dopamine (DA) (p < 0.01), and the percentage in Neurobasal/B27 is greater than in HD, RF, FCS or DA (p < 0.001).